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Experiments were conducted to study effects of steroidal carbamate derivatives upon mycelial growth and aflatoxin production by Aspergillus flavus ATCC 15517. The basal medium was supplemented with various concentrations of these compounds and inoculated with spores. The developing cultures were incubated for 11 days at 28℃ without agitation. Aflatoxins were extracted with chloroform, separated by thin layer chromatography, and quantitated by ultraviolet spectrophotometry. At a concentration of 50 ㎎ per 30 ㎖ of medium., stigmasteryl-N-(2-chloroethyl) carbamate, cholesteryl-N-(2-chloroethyl) carbamate, 5α-cholestan-3-one-oximino-N-(2-chloroethyl) carbamate and β-sitosteryl-N-(2-chloroethyl) carbamate were the most effective in reducing aflatoxin production by Aspergillus flavus. However, cholest-4-ene-3-one-oximino-N-(2-chloroethyl) carbamate, at a concentration of 100 ㎎ per 30 ㎖, significantly decreased aflatoxin production. There was no significant inhibition of mycelial growth by the addition of the various concentrations of these compounds.
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